miR-205a mediated suppression of CDH11 disrupts Wnt/β-catenin signaling and impairs chondrocyte differentiation.

At the gene expression level, qPCR analysis revealed that miR-205a mimic treatment significantly reduced Wnt4a mRNA levels, showed a trend toward downregulation of β-catenin , and upregulated GSK-3β , though the latter change did not reach statistical significance.

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Conversely, miR-205a inhibition upregulated β-catenin protein expression, with GSK-3β showing an opposite trend that also failed to reach statistical significance (Fig. 3B, C ).

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Notably, the protein levels of CDH11 showed a decreasing trend in the mimic group and an increasing trend in the inhibition group, but the changes were not significant (Fig. 3D, E ). qRT-PCR analysis revealed that co-transfection of miR-205a mimic with thiram dramatically increased miR-205a expression by approximately 25,000-fold (Fig. 3F ).

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qRT-PCR analysis revealed an upward trend in miR-205a expression in primary chondrocytes treated with thiram, though this increase did not reach statistical significance (Fig. 1C ).

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