Exposure of GT 1 -7 cells to H3 resulted in an increase in GnRH release at 1 h that became highly significant at a concentration of 1,000 nM H3 [100 ± 10.1 basal vs. 247.2 ± 8.3 (1,000 nM H3), data shown as %basal, P < 0.001 vs. serum-free medium] ( Fig. 6 ).
Although testosterone was increased 30 min following injection, this effect failed to reach statistical significance [9.97 ± 1.79 nmol/l (vehicle) vs. 19.19 ± 3.53 nmol/l (H3), P = 0.056], ( Fig. 2 B ).
A single intracerebroventricular injection of H2 to male Wistar rats showed a trend toward increased plasma LH 30 min postinjection, but this did not achieve statistical significance ( Fig. 1 A ).
Following intracerebroventricular H3 administration, total plasma testosterone rose, mirroring changes in circulating LH concentrations, although these changes did not reach statistical significance.