IgG Antibody Responses to the <i>Aedes albopictus</i> 34k2 Salivary Protein as Novel Candidate Marker of Human Exposure to the Tiger Mosquito.

A highly significant increase of both anti-al34k2 IgG1 and IgG4 levels was observed in PD2 by pairwise comparisons between paired samples ( n = 68, p < 0.0001; Figure 4B ); instead, only IgG4 levels showed a weakly significant increase after summer when all samples were considered ( p = 0.0326, Figure 4A ).

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IgG antibody responses to SGE and al34k2 showed a trend to decrease with age.

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Spearman's rank correlation analysis indicated a clear trend of antibody responses to decrease with age for both Padova surveys, especially when considering the anti-SGE IgG responses; on the contrary, in Belluno a weakly significant negative correlation was only found for the anti-al34k2 IgG responses in the BL2 survey ( Table 2 , Supplementary Figure 3A ).

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