Barely Significant

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Regardless of the specific countries, among which a huge variability was observed and where geographical effects are difficult to disentangle from those related to population, diagnostic matrix, and approach, an overall trend toward an increasing detection frequency in pet shops and breeding facilities was observed.
The latitude-year distribution plot ( Figure 5 B) shows that while the proportion of Blue Mackerel fluctuated across latitudes from year to year, it displayed an overall trend of “decreasing proportion with increasing latitude”.
Alongside the results obtained through one-way repeated measurements ANOVA on the ELISA data, an ANOVA applied to the spectral areas of the cortisol band obtained through Raman spectroscopy also showed statistically significant differences among the three experimental conditions (PRE, POST, POST60), displaying an overall trend that was consistent with that observed using the ELISA method.