Only Lactobacillus showed a highly significant negative correlation with starch ( P < 0.001) but showed a highly significant positive correlation with acidity and moisture ( P < 0.001).
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However, we still found highly significant differences across host clades and species when we analyzed the runs separately (by blocking), or together with run as an interactive effect (all P < 0.001, Supplementary Table 2 ).
The GLM revealed highly significant effects of organic matter duration on TC accumulation ( F = 30.23, p < 0.001), with crop type showing a secondary influence ( F = 6.55, p < 0.05; Supplementary Table S1 ).
Carbonate Chemistry Observations showed that DIC ( Figure 2A ) and A T ( Figure 2B ) were consistently higher in the muddy sediment compared to the sandy sediment across all treatments, and sediment was highly significant for both A T ( p < 0.001) and DIC ( p < 0.001).
Finally, the correlation analysis between gut microbiota and metabolites also confirmed that the abundance of the genus Alistipes exhibited a highly significant negative correlation with lithocholate sulfate levels ( p < 0.001).
For the bacteria, the interaction effect between biochar addition and time was extremely significant ( p < 0.001).
The Tissue × Origin interaction term was highly significant for all three enzymes ( P < 0.001), indicating that the immune expression of B. lenok under different growth environments is tissue-specific. 3.5 Analysis of antioxidant parameters As shown in Figure 5 , hepatic CAT activity and GSH content in the wild group were significantly higher than in the farmed group ( P < 0.01).
Further Unweighted_unifrac Adonis analysis indicated there was a highly significant independent distribution of gut microbiota between the P group and N group ( p = 0.001) ( Figure 2B ). 3.3 Functional prediction of gut microbiota 3.3.1 Functional prediction in gut microbiota based on MetaCyc metabolic pathway As shown in Figure 3 , the MetaCyc database was used to predict the primary metabolic functions of the gut microbiota in groups N and P.
As shown in Figure 10A at the genus level Candidatus_Scybalousia showed a highly significant positive correlation with the COL11A1 gene ( p < 0.001), Candidatus_Pelethosoma showed a significant positive correlation with the COL11A1 gene ( p < 0.01) and Candidatus_ Fimihabitans, Mesoplasma, Paramaledivibacter, Xylanibacillus, Zarconia, Gallionella were positively correlated with COL11A1 ( p < 0.05).
hydrophila isolates were statistically highly significant ( p < 0.001, Fisher’s exact test).
lata , pure cultures that were exposed to 79 at.% Cu surface, a linear increase of the metabolic activity was followed by an exponential rise after 18 h of incubation, resulting in a highly significant ( p < 0.001; 20 h into alamarBlue: 79 at.% Cu 58%; PC 113%) difference compared to the positive control.
For Escherichia coli , the interaction effect was not significant ( p = 0.0907), although both treatment and volume independently showed highly significant effects ( p < 0.001).
In the fungal community, both indices of the Tb groups exhibited lower values than those of the CK groups, particularly in the Shannon index, which displayed highly significant differences across all regions ( p < 0.001).
Welch’s t -test showed an extremely significant difference in alpha diversity between water and sediment samples ( p < 0.001) ( Figures 8A – D ).
On a relative basis, the FBA gene of the F10-D strain presented extremely significant upregulation on days 5 and 7 in YPD + Fru medium, with a 1.4-fold increase on day 5 ( p < 0.001) compared to that in the YPD group; the FBA gene of the T17-D strain in YPD + Fru medium was upregulated on days 1, 3, and 5, with a 1.5-fold increase on day 5 ( p < 0.05); the FBA gene of the TF15-A strain was extremely significantly upregulated on days 1, 3, 5, and 7, and the expression level was upregulated almost 2.5-fold ( p < 0.001) on day 5.
The results were presented as means +/± standard deviation (SD), and the difference between means is considered significant if ∗ P < 0.05, very significant if ∗∗ P < 0.01, and extremely significant if ∗∗∗ P < 0.001.
After 60 d of ensiling, pH exhibited a highly significant negative correlation with LA content ( r = -0.89, p < 0.001) and a strong significant positive correlation with AN content ( r = 0.86, p < 0.001).
In OFT, reduced locomotor activity/exploratory behavior was evidenced by a highly significant decrease in the number of rearings ( p < 0.001; Figure 7B ), line crossings ( p < 0.001; Figure 7A ), and time spent in inner squares ( p < 0.01; Figure 7C ) in the infected group as compared to the control group.
The differences were considered to be statistically significant if P ≤ 0.05 or 0.001 < P ≤ 0.01 and were considered extremely significant if the P < 0.001.
Such a difference in the response between the two cultivars, i.e., the interaction between cultivar and plant disease status, was highly significant ( P < 0.001).
The biochar amendment exhibited highly significant positive effects on multifunctionality ( P < 0.001), whereas the changes in ecosystem multifunctionality had negative associations with the average of the indicators of soil biodiversity ( P < 0.05) ( Supplementary Figure 4 ).
Viral abundance increased for all treatments over time ( f = 48.02, p < 0.001) and between enrichment steps ( f = 12.66, p < 0.001) with a highly significant interaction ( f = 6.78, p < 0.001).
It showed a highly significant ( p < 0.001) and strong provincialism, that explained roughly 30% of the total variation of community composition for bacteria from all three size fractions of the plankton (adjusted R 2 value between 0.32 and 0.30).
Only the highly significant ( p < 0.001) mutual relationships were retained.
NDF concentration showed a highly significant positive correlation with WSC concentration ( p < 0.001) and pH ( p < 0.01), but a significant negative correlation with LA concentration ( p < 0.05).