Barely Significant

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Under Mg-deficiency, N, P, and Mg concentrations in the blades displayed a decreased trend with increasing leaf age; K, Ca, Fe, Cu, and Zn concentrations in the blades displayed an increasing trend with increasing leaf age; Mn concentration was similar among the blades of lower, middle and lower leaves; B concentration was higher in the blades of lower leave than that in the blades of upper and middle leaves.
Further, for all the 15 genes studied, the mutants as well as the WT showed an increasing trend in gene expression with the progression of time under incubation, irrespective of whether they are up or downregulated, i.e., the expression of the genes increased as the time under dark condition increased and this rate of increase was the highest in the WT followed by SGM-3, while SGM-1 and SGM-2 were at par showing the least rate of increase ( Figure 3 and Table S2 ).