Barely Significant
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extremely significant

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extremely significantP < 0.010.2× alphaqualified
The values, means ± SD (MS: n = 12, FH: n = 21), in the same row with different lowercase showed statistically significant differences ( P < 0.05) and the values labeled with different capital letters represent extremely significant difference ( P < 0.01) according to the Kruskal–Wallis test, the same as below. 2.5.
extremely significantp < 0.010.2× alphaqualified
LSD and Duncan's multiple range tests facilitated multiple comparisons of means, categorizing differences as extremely significant ( p < 0.01), significant ( p < 0.05), or non-significant ( p > 0.05) based on p values. 3 Results 3.1 Growth performance The initial body weight comparison indicated no significant differences between the CON, AGF, MN, and TW groups ( p > 0.05).
extremely significantp < 0.010.2× alphaqualified
At the age of 84 days, extremely significant interaction effects of the swimming pool conditions and floor types were observed for eye cleanliness, feather cleanliness, and breast blister scores ( p < 0.01), while the interaction effect of feather quality showed a significant difference ( p < 0.05).
extremely significantp value < 0.010.2× alphaqualified
Statistical analysis of the relative mRNA expression level between transcriptomic data and qPCR data was performed using Student’s t-test with SPSS 26.0 (v26) software, and the differences were considered significant when the p value < 0.05 (*) and extremely significant when the p value < 0.01 (**). 5.