Rabphilin-3A negatively regulates neuropeptide release, through its SNAP25 interaction.

Although not significant we observe a clear trend towards bigger neurons compared to WT.

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In agreement with previous findings ( Ahnert-Hilger et al., 1996 ; Grosse et al., 1999 ; Osen-Sand et al., 1996 ), we find that TeNT expression did not affect neurite length of WT neurons, but showed a trend toward shorter neurites in RPH3A KO neurons.

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The increase in neurite length may partially depend on regulated secretion, as TeNT expression showed a strong trend toward reduced neurite length to WT levels ( Figure 6 ).

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While the difference between KO neurons +/- TeNT did not reach statistical significance.

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