Barely Significant

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Additionally, compared to the T1 group, the mRNA expression level of 4e-bp1 (a negative regulator of protein synthesis metabolism) was significantly reduced in the T4 group, while a decreasing trend in expression was observed in the T2 and T3 groups; however, these differences were not statistically significant. 4.
However, on day 28, we observed an increasing trend in the relative abundance of Firmicutes , which could be explained by the effect of RMD, as indicated by previous research [ 43 , 44 ], Another possible factor is the production of lactate by Bifidobacterium longum , which can be utilized by members of Lachnospiraceae , thereby stimulating their proliferation [ 45 ].