The levels of ethers showed an overall increased trend, and the contents of ethers increased sharply in the 20th year of storage, with a maximum of 11.57%, and the contents of alkanes and acids showed a decreasing trend overall. 3.3.
Correspondingly, the growth rates of both fungi displayed a decreasing trend with 0.75–1.25% of CO concentrations, but this trend was reversed at higher CO levels.
Specifically, the inhibitory efficacy of the CO–C:G film against RM01 exhibited an increasing trend, ranging from 0 to 66% with a reduction in the fungal growth rate from 18.75 ± 0.00 cm 3 /day to 8.17 ± 0.05 cm 3 /day as the CO concentration increased from 0.75–5.0%.
While the lower dose (20 μg/mL) did not reach statistical significance, a decreasing trend in DNMT1 expression was observed compared to the H 2 O 2 and vehicle-treated group.
With the extension of the fermentation time, within the range of 0–10 d, the amount of CheA biosynthesis by each strain also showed an increasing trend.
Similarly, the MDA content showed a trend of first increasing and then decreasing ( Figure 4 b), and the nisin treatment could significantly reduce the MDA content of the fresh-cut pumpkins, indicating that nisin treatment could inhibit the occurrence of membrane lipid peroxidation.
Additionally, an increasing trend in AV, POV, and TBARS contents was observed after the freezing process, with lipid oxidation being significantly higher in the −18 °C group compared to the −25 °C group ( p < 0.05).