Excerpts

The reduced model was significant and explanatory ( p < 0.0001, adjusted r 2 of 0.57) and contained nine predictor variables, which were all individually significant ( p < 0.05) or highly significant ( p < 0.0001), suggesting the importance of a suite of factors in predicting abundances ( Table 1 ).

As shown in Figures 3A,B and Table 2 , while both gO GT1c cysteine mutants, gO GT1c-C218S and gO GT1c-C343S, displayed a similar infectivity for HFFs as their parental strains GT1c_1 and GT1c_2, the mutation of C336S in gO GT4 led to reduction in infectivity, which was significant when compared to GT4_1 ( p = 0.0025) and highly significant when compared to GT4_2 ( p < 0.0001).

This observation was confirmed by the two-way PERMANOVA, which crossed swabbed region with health, stating that there were highly significant differences between regions and globally between health states (pseudo- F = 22.241, p -value = 0.0001, pseudo- F = 10.373, p -value = 0.0001, respectively) (Supplementary Table S1 ).

The difference in ciliostasis between the strain-GD-infected group and the control group was highly significant ( p < 0.0001).

sclerotiorum populations from the four different countries with the R ST fixation index statistic highly significant ( P < 0.0001) for pairwise combinations of England/Norway ( R ST = 0.094), England/Australia ( R ST = 0.186), Scotland/Norway ( R ST = 0.068), Scotland/Australia ( R ST = 0.152), and Norway/Australia ( R ST = 0.198).

The statistical difference in number of accessory genes between MRSP and MSSP isolates as measured using Welch’s t -test was highly significant ( p < 0.0001), with MRSP isolates harboring an average of more than 150 accessory genes compared to their MSSP counterparts ( Figure 3C ).

As shown in Figure 3B , the addition of CypA or CypB resulted in a highly significant improvement ( p < 0.0001) in the refolding of the acid-denatured GFP confirming their true enzymatic activity.

However, increasing arsenate concentration had a highly significant effect on normalized growth rate ( p < 0.0001; Figure 3 A).

Fisher's exact test indicated highly significant differences across all groups ( p < 0.0001), reinforcing the potential of epitope 5 as a reliable diagnostic marker.

All three analyses showed highly significant interactions between PST and light ( p = 0.0001, p = 0.0002, p = 0.0009, for PST content, production, and C fraction, respectively), a significant interaction between lytic toxicity and whether the strains contained PST ( p = 0.0157 for PST content), and significant effects of lytic toxicity on growth ( p = 0.0125, p = 0.0175, for PST production and C fraction, respectively).

The antagonistic strains had a highly significant effect on fungal growth ( F -value = 26333.5, p < 0.0001) ( Figures 2 , 3 ).

The 1-way ANOVA showed that differences between individual cells of the parallels Nitrate were highly significant for 15 N- and 13 C-enrichments ( p < 0.0001).

For instance, decreasing the incubation temperature from 37 to 30°C or room temperature, had a highly significant effect on the total aerobic count for samples collected from the low care zone ( p < 0.0001).

A two-way ANOVA analysis showed that the ratio of activated carbon to AC-1 had a highly significant impact on the recovery rate of immobilized AC-1 strains over 120 days of storage ( F = 6.842, p < 0.0001), while the variance analysis results between the bacterial suspension and fermentation broth were significant ( F = 3.274, p < 0.01) ( Figure 1 ).

ANOVA revealed highly significant differences among treatments ( p < 0.0001), and Tukey’s post hoc test confirmed that the combination exhibited significantly higher inhibition compared to salicylic acid ( p < 0.001) and ibuprofen ( p < 0.001) alone, while ibuprofen was also significantly stronger than salicylic acid ( p < 0.001).

For plipastatins, this effect compared with that of the control without lipopeptide was statistically significant ( p = 0.0023, using the Dunn's multiple comparison test) for 300 mg.L −1 of the sample and extremely significant ( p < 0.0001) for the higher concentration (500 mg.L −1 ) of plipastatins.

Considered separately for Udupi ( Figures 5A,B ) and Shimoga ( Figure 5C,D ), the differences between loads detected in the monsoon season in Udupi (3.47 × 10 3 ; IQR: 1.13 × 10 3 – 1.55 × 10 4 ) and Shimoga (5.02 × 10 3 ; IQR: 3.29 × 10 3 – 1.78 × 10 4 ) compared to the summer season in Udupi (1.55 × 10 3 ; IQR: 1.35 × 10 2 – 4.99 × 10 3 ) and Shimoga (9.18 × 10 2 ; IQR: 5.37 × 10 1 – 1.80 × 10 3 ) were again highly significant ( p = 0.0001 and p < 0.0001, respectively).

Subsequent validation revealed a highly significant positive correlation ( r = 0.91; p < 0.0001) between RNA-seq and RT-qPCR results indicating a high reliability of the RNA-seq data.

Statistical analysis using one-way ANOVA confirmed that the differences in biofilm formation between treated and untreated groups were highly significant ( p < 0.0001 for Δ agrA and MRSA).

Likewise, biofilm amount of each strain did not change significantly during assay ( P > 0.05), whereas highly significant ( P < 0.0001) differences in biofilm biomass were found among the assayed strains.

The distribution of the clade specific patterns was extremely significant associated with the tree topology and geographic location of the strains. ∗∗∗ = ( p < 0.0001).

IE2 positive cells showed a highly significant ( p < 0.0001) down-regulation of CD83 surface expression ( Figure 5A , white bar) which could be prevented by the addition of the proteasome inhibitor MG-132 ( Figure 5A , gray bar).

The quadratic model Y = 1.08 + 0.092× 1 + 0.045× 2 + 0.15× 3 –0.13X 1 X 2 –0.14× 1 2 –0.23× 2 2 –0.20× 3 2 was highly significant ( p < 0.0001) and lacked-of-fit was not ( p = 0.197).

The inhibition effects of immune sera had a correlation with dilutions of anti-rTsDNase II-1 serum ( r = -0.881, P < 0.0001) and anti-rTsDNase II-7 serum ( r = -0.891, P < 0.0001) and exhibited a decreasing trend with a serum dilution increase ( F 1 = 20.749, F 2 = 27.655, P < 0.0001).

wound infection biofilm antimicrobial antiseptic hypochlorous acid human plasma biofilm model wound irrigation chronic wound internal grant program of the Faculty of Health at Witten/Herdecke University, Germany IFF2018-61 German “Initiative Chronische Wunde e.V.” (ICW e.V.) Both reference substances, OCT/PE and PHMB-based irrigation solution, achieved a highly significant reduction of P. aeruginosa (4.77 ± 1.41 and 5.25 ± 0.93 log 10 steps, respectively; p < 0.0001), S. aureus (6.18 ± 0.00 and 4.82 ± 1.36 log 10 steps; p < 0.0001), and MRSA (both 6.18 ± 0.00 log 10 steps; p < 0.0001) within 30 min of exposure ( Figures 2A – C ).