Barely Significant

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euphratica population age structure follows a pyramid structure with an increasing trend ( Han et al., 2013 , 2014 , 2017 ), while in the middle and lower reaches, the population structure exhibits an inverted pyramid shape, with a declining trend ( Xu et al., 2016 ; Zhou et al., 2018 ).
Compared with SC, the relative abundance of Cryptococcus in CK exhibited an extremely significant decline, the relative abundance of T etraclaudium , Alternaria , Clonostachys , and Chaetomium decreased significantly, and the relative abundance of Pseudonymnoascus increased significantly ( Supplementary Figure 2 ).
The qRT-PCR results demonstrated that the expression levels of lignin biosynthesis genes including PtrPAL4 , PtrC4H1 , PtrC3H3 , Ptr4CL5 , PtrCCoAOMT3 , PtrCOMT2 , PtrCCR2 , PtrCAld5H2 , and PtrCAD1 , lignin polymerization genes including PtrLAC19 , PtrLAC21 , PtrLAC26 , and PtrLAC41 , and hemicellulose biosynthesis genes including PtrGT43A , PtrGT47C , and PtrGT8F , increased at extremely significant levels, while the expression levels of the cellulose biosynthesis genes such as PtrCESA4 , PtrCESA7 , and PtrCESA8 , had no obvious increases in PtrWOX13A transgenic lines compared with WT ( Figure 6A ).