Barely Significant
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a trend toward significance

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a trend toward significancep = 0.0591.2× alpha
Cardiovascular risk factors were not particularly represented in the study population, with hyperlipidemia and arterial hypertension being the most frequent; both were more common in men, with arterial hypertension reaching a statistically significant difference ( p = 0.002), while dyslipidemia showed a trend toward significance ( p = 0.059).
a trend toward significancep = 0.0591.2× alpha
Lumbar spine tumors (n = 26) showed a trend toward significance when compared with non-lumbar tumors (n = 67) (p = 0.059, between-group comparison), but this did not reach the conventional threshold for statistical significance (Table 13 ).
a trend toward significancep = 0.0591.2× alpha
However, there was a trend toward significance for neoadjuvant treatment ( p = 0.059) and response to neoadjuvant therapy ( p = 0.065), with higher mean miR-3173 expression observed in patients who received neoadjuvant treatment and those with response scores of 4-5. miR-3173 expression was also higher in grade III tumors (9.62 ± 7.11) compared to grade II (3.93 ± 4.53) and grade I (1.04) tumors, although this difference was not statistically significant ( p = 0.174).
a trend toward significanceP = 0.061.2× alpha
To complement the supervised data, we also utilized an unsupervised method called PCoA, which also showed dissimilarity between AMD and control subjects, with a trend toward significance by PERMANOVA analysis ( P = 0.06) after adjusting for age ( Fig 3 B).