Barely Significant

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For the flavanols catechin, epicatechin, procyanidin B2, procyanidin C1, and cinnamtannin A2, an interesting trend emerged: the chocolate filling produced with palm oil exhibited significantly lower levels of all five quantified flavanols compared to fillings made with sunflower oil, crystallised sunflower oil with press cake (COP), and crystallised sunflower oil with press cake and emulsification (COPE).
At the genus level, the relative abundance of norank_f__Muribaculaceae , Lachnospiraceae_NK4A136_group , Alistipe, Parabacteroides , Bacteroides , and Prevotellaceae_ UCG-001 in the CTX group showed a decreasing trend, while the abundance of Lactobacillus and unclassified_f__Lachnospiraceae significantly increased.
Among them, the ROAV of 1-octen-3-ol gradually decreased with the extension of cooking time, and the ROAV of 2-pentylfuran and methyl cinnamate showed an increasing trend, which indicates that long-term cooking resulted in loss of the mushroom aroma of T. matsutake soup, but it would bring some floral and fruity aroma.