In all logistic regression models, the overall effects of sampling region and year on the prevalence of the individual agents were highly significant ( P < 0.001 for all).
Excerpts
The results were presented as means +/± standard deviation (SD), and the difference between means is considered significant if ∗ P < 0.05, very significant if ∗∗ P < 0.01, and extremely significant if ∗∗∗ P < 0.001.
This test found a highly significant difference (χ 2 = 44.113, 6 d.f., P < 0.001) driven by the existence of two non-overlapping groups (Tukey test, P ≥ 0.171).
In RG and IS, we furthermore observed a decreasing trend of aerobes and facultative anaerobes ( p < 0.001 and p = 0.002, respectively) and an increase of anaerobes during the observational period ( p < 0.001 for both, Figure 6 ).
Structural equation modeling further revealed that tree mycorrhizal dominance and microbial biomass were the primary factors alleviating microbial nutrient limitation: microbial biomass and mycorrhizal dominance showed significant negative effects on vector length (path coefficients −0.71 and 0.33, p < 0.01), whereas mycorrhizal dominance exerted a highly significant positive effect on vector angle (path coefficient 0.70, p < 0.001).
For example, the divergence in fungal community composition at this salinity threshold was highly significant in dataset 2 (Permanova; P = 0.001, Figure 3B ).
The Tissue × Origin interaction term was highly significant for all three enzymes ( P < 0.001), indicating that the immune expression of B. lenok under different growth environments is tissue-specific. 3.5 Analysis of antioxidant parameters As shown in Figure 5 , hepatic CAT activity and GSH content in the wild group were significantly higher than in the farmed group ( P < 0.01).
Agricultural practice had a highly significant effect on the soil microbial biomass C ( p < 0.001), but water content and its interaction with agricultural practice not (Supplementary Table S1 ).
AP showed a highly significant positive correlation with Variovorax ( p < 0.001).
Kaplan–Meier analysis confirmed highly significant differences in survival across groups (log-rank test, p < 0.001).
After 60 d of ensiling, pH exhibited a highly significant negative correlation with LA content ( r = -0.89, p < 0.001) and a strong significant positive correlation with AN content ( r = 0.86, p < 0.001).
There were highly significant differences among the three experimental groups ( P < 0.001, Figure 9A ).
However, not all strains displayed the same response to sterol type, as the interactions between sterol type and strain were highly significant for all variables (value of p <10 −3 ), except tCO 2 _30 (not significant).
Trichosporiella exhibited a highly significant positive correlation with Apigenin ( P < 0.001), while Cadophora showed a highly significant positive correlation with Hyperoside ( P < 0.001).
Differences between groups were considered significant if p < 0.05 (indicated with *), highly significant if p < 0.01(indicated with **) and extremely significant if p < 0.001 (indicated with ***).
One-way ANOVA detected highly significant treatment effects for the four β -lactams AML ( F = 19.54, p = 0.001), AUG ( F = 19.04, p = 0.001), PP ( F = 29.72, p = 0.0002) and CTX ( F = 19.60, p = 0.001); no differences emerged for cefpirome, carbapenems or quinolones ( p > 0.05) ( Supplementary Figure S2d ).
As depicted in Figure 5C , fermentation exerted a highly significant effect ( p < 0.001) on differential metabolites involve in ABC transporters, aminoacyl-tRNA synthesis, and ascorbic acid and aldonic acid metabolism pathways.
Mantel correlation further indicated that while the bacterial community structure of the FL and PA size fractions were significantly different (ANOSIM test: R = 0.37, p < 0.001; Supplementary Table S9 ), there was a highly significant positive relationship between the two size fractions ( r = 0.92, p < 0.001; Partial mantel test) ( Figure 8 ).
The analysis indicated a highly significant disparity between the two groups (χ 2 = 45.0, df = 1, p < 0.001), confirming that Z2-1 inoculation markedly promoted the establishment of symbiosis between P. umbellatus and A. gallica .
This difference was significant as early as 24 h after inoculation ( p < 0.01, Wilcoxon test), and highly significant after 30 h of incubation ( p < 0.001, Wilcoxon test).
Association study by Statistical analysis Statistical analysis revealed highly significant ( p < 0.001) positive associations among biofilm formation, antibiotic resistance, and virulence gene ( invA ) prevalence in Salmonella isolates.
In this study, there were significant ( p < 0.05) differences in MK content between day 22 and 28 in C1, C2, and C6, while extremely significant ( p < 0.001) differences were observed in C3, C4, and C5, suggesting the suitability of C1, C2, and C6 as fermentation substrates for appropriately shortened-term fermentation processes.
Propionic acid was less abundant than butyric acid in both animal-diet ( F = 0.3, P = 0.62, non-significant) and plant-diet ( F = 47.8, P < 0.001, highly significant) samples.
“ns” indicated no significant difference; P < 0.05 was considered as a significant difference; P < 0.01 was moderately significant; P < 0.001 was extremely significant. *, P < 0.05; **, P < 0.01; ***, P < 0.001 using a one-way analysis of variance (ANOVA) followed by Tukey’s HSD test.
LEfSe was carried out with the linear discriminant analysis (LDA) threshold score of 3.00, to find which specific fungal communities caused the highly significant differences ( p = 0.001) in fungal communities between PM samples at different depths ( Figures 5C,D ).