Barely Significant

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3.3 High-throughput sequencing of 16S rDNA 3.3.1 Venn and α-diversity analysis of gut microbiota A Venn analysis showed a total of 679 operational taxonomic units (OTUs) in the two groups ( Figure 4A ), of which each group had unique OTUs, and the OTUs in the OVX group showed a decreasing trend compared with the sham group.
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3.7 Correlation analysis of soil carbon, nitrogen, and phosphorus cycling functional genes with environmental factors and functional microorganisms Heat map analysis of soil carbon, nitrogen and phosphorus cycling genes correlated with soil physicochemical properties and enzyme activities ( Figure 9 ), korA/B , which is associated with carbon fixation, was positively correlated with S-β-GC, and TC and SOM had high concordance, and negatively correlated with most of the carbon cycling genes ( Figure 9A ); GDH2 , norB were highly significant negatively correlated with TC, SOM, AN and S-LAP, glnA, nirB and gltB were all significantly negatively correlated with SOM, where gltB and AN were highly significantly negatively correlated, and nirA was the opposite, but TN showed a weak correlation with the nitrogen cycle; for the soil phosphoru
The exceptions, which were barely statistically significant, were (i) a shorter recurrence interval for isoclonal group A isolates (10 pairs: median interval, 45 days) than for isoclonal group C isolates (four pairs: median interval, 90.5 days) ( p = 0.02), and for fluoroquinolone-resistant isoclonal isolates (59 pairs: median interval, 56 days) than for fluoroquinolone-susceptible isoclonal isolates (60 pairs: median interval, 65 days) ( p = 0.04).
In general, the CP content in silage shows a decreasing trend as the anaerobic fermentation process advances, and the biological activity of adherent microorganisms affects the degree of degradation of CP content after the addition of exogenous additives ( He et al., 2018 ).
Data from the ECDC latest surveillance report ( European Centre for Disease Prevention and Control, 2022 ) showed indeed an increase in the population weighted mean MDR percentage over the period 2018–2022 and an increasing trend in infections caused by CRPA and carbapenem-resistant Klebsiella and Acinetobacter spp.
3.3 Volatility analysis Table 5 displays the volatility analysis results, with the correlation coefficients ( r -value) and corresponding significance ( p -values) of the relationships between the performance, digestibility and slurry variables studied and bacterial genera found to be significant or near to significance.
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P < 0.05 (*) indicates a significant difference, while P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****) indicates a highly significant difference. 3 Results 3.1 DIC inhibits PRRSV infection in vitro We conducted an experiment to assess the cytotoxicity of DIC in Marc-145 cells using a Cell Counting Kit-8 (CCK-8) assay ( Figure 1A ).