Barely Significant

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There was a marginally non-significant tendency for first litters of iteroparous females to develop more slowly than litters of semelparous females (age at eye opening 66±0.8 days in first litters, 64±0.8 days in both second litters and offspring of semelparous mothers; overall model: F 2,29 = 1.7, P = 0.19, difference in age at eye opening between litters with semelparous mothers and first litters of iteroparous mothers: t = −1.9, df = 29, P = 0.07; difference between first and second litters of iteroparous mothers t = −1.2, df = 29, P = 0.24).
Analysis of the amino acid changes characterizing the selected mutants highlights a clear trend towards the replacement of residues highly prone to oxidative damage, such as aromatic and aliphatic residues, with residues less prone, such as shorter chain aliphatic residues and polar residues ( Table S1 ).
Although there was a trend towards significance in the frequency of Th1 and Th17 cells in HKTC-immunized mice relative to control mice, this increase was not significant, and there was also no significant increase in Th2 responses, despite the detection of significant levels of IFN-γ + and IL-4 + antigen-specific bulk splenocytes in mice immunized with HKTC.