Barely Significant

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Biochar treatments were not consistent in their effects on rice leaf O 2 − content, which generally showed that rice leaf O 2 − content under B1, B2, and B3 treatments showed an increasing trend in both reproductive stages; the B2 treatment especially differed from the B0 treatment to a significant level in the heading stage ( Figure 3 B); however, the high-biochar treatment (B4) significantly decreased rice O 2 − content ( p < 0.05) under the N225 treatment at the heading stage and the N0 and N225 treatments at the filling stage, with O 2 − content under the treatments ( p < 0.05).
Specifically, there was an extremely significant correlation between the variation in DB content and the presence of DEMs 6-hydroxymelatonin, L-ornithine, Asp-Phe methyl ester, 4-hydroxy-3-methylbenzoic acid, β-cortolone, 3-methylcrotonylglycine, coenzyme Q2, 4-[2-(4-chlorophenyl) diaz-1-enyl]-2-methyl-6-(piperidinomethyl) phenol and D-threose, and significantly correlated with metabolites cytidine and 1-palmitoyl-Sn-glycero-3-phosphocholine, etc.