Barely Significant

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In addition, the number of lactone groups (-COOR), phenolic hydroxyl groups (-OH), and carboxyl groups (-COOH) on the surface of biochar gradually decreases as the temperature of biochar increases, while the number of carbonyl groups (-C = O) gradually increases, but the total amount of acidic oxygen-containing functional groups shows a decreasing trend, which is consistent with the FTIR analysis results of biochar.
Given the consistent trend in most of the datasets, we further investigated the abundance of m6A_WTAP + CD8 + T cells in different patients using immunofluorescence analysis of tissue microarrays; this revealed a decreasing trend of m6A_WTAP + CD8 + T cell infiltrates with a higher clinical stage (Fig. 7 c-f).