Barely Significant

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With the increase in the elastic modulus of the surrounding strata, the internal net height and height-to-span ratio of the tunnel generally show an increasing trend, while the external wall span, vault thickness, invert arch thickness, side wall thickness, and upper arch curvature generally show a decreasing trend.
Although non-significant, we observed a decreasing trend, in expression of genes encoding mitochondrial oxphos complex I (NADH dehydrogenase subunit 1 (nd1) ), Complex III (cytochrome b-c1 complex subunit 2, mitochondrial ( uqcrc2 )), complex IV (cytochrome c oxidase subunit 4 isoform1, mitochondrial, (cox4i1) ) and complex V (ATP synthase subunit beta, mitochondrial, ( atp5f1b) ).