Barely Significant

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When post-treatment samples were also classified according to therapy, a significant reduction in inverse Simpson’s ( p = 0.049) index was detected in paired t-test exclusively for samples from patients treated with a FED, while p -values were close to significance for Chao1 and Shannon’s H indices (0.056 and 0.075, respectively) (Fig. 1 D–F).
5 ) showed that the OD value of biofilm had an increasing trend in the control and 1/8MIC SH groups with the increase of incubation time, suggesting the strains adhered to the titanium surface and gradually formed biofilm; from 6 to 24 h, we observed significant differences of the OD values between any two time-points ( P < 0.01), while no statistical difference of OD value was found for S. epidermidis ATCC 35984 (Fig. 5 a) or MRSE 287 (Fig. 5 c) ( P > 0.05) and statistical difference was found for S. aureus ATCC 43300 (Fig. 5 b) ( P < 0.01) between 24 and 48 h.
Characterization of gene expression revealed an expected trend for both encapsulated and control spheroids—expression of pluripotency genes ( OCT4, SOX2, NANOG ) was high with cells in pluripotency (mTeSR) media at days 1 and 3 but then decreased after spheroids were transferred into endodermal differentiation media during subsequent 3 days (Fig. 6 B,C).