Excerpts

Acquisition and analysis of intersection targets of ROSA and CHIKV- The PPI analysis of the CHIKV disease target network revealed a total of 502 nodes and 1,386 edges, with a highly significant p-value of < 1.0e-16 ( Figure 2A ).

The resulting network comprised 14 nodes and 87 edges with a highly significant enrichment (p-value <1.0e–16), indicating nonrandom functional connectivity among apoptosis, inflammation, and oxidative-stress regulators.

After removal of disconnected nodes, the refined network consisted of 267 nodes and 1154 edges, with an average degree of 8.64 and a highly significant PPI enrichment ( P < 1.0e-16).

The network showed highly significant PPI enrichment (p < 1.0 × 10 −16 ), with an average node degree of 3.52 and a local clustering coefficient of 0.497, supporting the presence of a non-random and moderately clustered interaction structure.

Overall, a test of independence between the risk score tertiles and the potentially AAMR CNV count classes shows a highly significant association ( P < 1 × 10 −16 ) for both Fisher's exact test and a χ 2 test.

Furthermore, we validated rhythmicity using two alternative detection methods [RAIN (Thaben & Westermark, 2014 ) and ARSER (Yang & Su, 2010 )], which showed a highly significant overlap between the groups ( Appendix Fig S1G ; two‐sided Fisher's exact test, P < 10 −16 for every pairwise comparison).

The correlation between HNRNPK binding events and nuclear enrichment was highly significant when controlling for transcript length and expression levels (R=0.2, P<10 -16 in HepG2 cells and R=0.07, P<10 -16 in K562 cells).

Using STRING analyses, we identified a highly significant protein–protein interaction (PPI) enrichment ( P = 10 −16 ) only for the downregulated genes of H2A.Z.1‐depleted cells.

Changes in mean activation magnitude in both regions between unscaled and scaled t-values are of the order of 10% of unscaled activation magnitude and are highly significant (a t-test over all voxels yields a p-value of p < 10 − 16 ).

In contrast, highly significant differences were observed between promoter usage in ΔCTCF-HPV18 episomes compared to HPV18 following keratinocyte differentiation (p < 1E-16).

We do detect a highly significant enrichment of marine-freshwater differentially expressed genes, as predicted ( P < 10 −16 ).

However, while the difference between term and moderate to late preterm TREC was significant but not meaningful, 108 (70–170) vs 101 (64–164) median TREC ( p = 0.0017), the differences between term and extremely preterm, 49 (29–92), or very preterm, 88 (52–149), were both highly significant ( p = 1E −16 , p = 4E −6 , respectively) and meaningful.

They were not identical to the ones identified in each disease individually, but showed a highly significant overlap in both cases ( p < 1E−16, χ 2 -test).

STRING analysis revealed a highly significant enrichment of protein–protein interactions (PPI enrichment p < 1.0 × 10 −16 ), confirming that the identified proteins are functionally connected rather than randomly associated.

The resulting network comprised 332 nodes and 1181 edges, with a highly significant PPI enrichment p -value < 1.0 × 10 −16 , indicating a non-random and biologically meaningful set of interactions.

As illustrated in Figure 3 , there was a highly significant correlation between the genetic and transcriptome covariances for this set of genes ( r = 0.035, P = 1.0 × 10 −16 ; Figure 3 ).

Notably, only proteins associated with our list of cKO- Eed -MADM–specific DEGs, but not sparse Eed -MADM–specific DEGs, formed a highly significant and densely connected network (PPI enrichment P value < 1 × 10 −16 ) (fig.

However, the overlap between the two categories of the intergenic transcribed regions is highly significant (P-value < 10 -16 , chi-square test)." Contributor Information Philipp Kapranov, Email: philippk08@gmail.com.

The global mediation test reveals a highly significant result ( p < 10 − 16 ) and low neutralization rate (21%), confirming that CT imaging strongly mediates the effect of PRS on FEV1.

Moreover, pairwise Levene’s tests for equality of variances between ancestry tracts were all highly significant ( p < 1 × 10 −16 ).

By enhancing the confidence levels of the PPI to the highest level (0.9), the number of edges decreased to 2534 and the node degree average to 8.11, but the PPI enrichment remained highly significant ( p < 1.0 × 10 −16 ), underscoring the quality of the proteomic data.

Of these, 1495 genes are detectably expressed in our lines, and 1079 (72.2%) are classified as DE between the species in both data sets (a highly significant enrichment; χ 2 p < 10 −16 ).

This difference between MS patients and non-MS controls is highly significant ( p < 10 − 16 ) .

The overlap between our set of 2,407 genes and this set of 1,092 genes was highly significant (p<1x10 16 , Fisher’s exact test, S2B Fig ) and, since RNA-seq is typically more sensitive than microarray analysis [ 31 ], we proceeded with the larger gene set.

In other words, organisms sampled from a defined skin habitat were more similar to each other in 16S sequence than expected for a global background; this enrichment was statistically highly significant (p<<10 −16 , one-sided Mann-Whitney-U test, see Table S1 ).