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“showed a trend”

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showed a trendP < 0.102.0× alpha
As demonstrated in Figure 2(g) (NMI results) and Figure 2(h) (TRE results), generating landmarks on anatomical curves (no matter the landmarks were optimized or not) either significantly improved registration accuracy ( P < 0.05, paired t -test) or showed a trend towards improvement (0.05 ≤ P < 0.10) in all image pairs compared to manually selecting discreet landmarks.