Barely Significant

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The genomic relatedness G-matrix heatmap ( Figure 2 B) showed a trend consistent with the IBS distance matrix: 55,904 pairs (99.927%) had G-matrix values less than 0.2 (green regions), reflecting moderate genetic relatedness among most individuals, while only 0.004% of pairs had G-matrix values greater than 0.6 (orange regions), indicating very few closely related individuals.
highly significantqualifiedgold
From an antimicrobial-reduction standpoint, these findings are highly significant, and by selectively enriching beneficial taxa, enhancing SCFA-mediated energy supply, and accelerating microbiome stabilization, FOS supplementation may reduce susceptibility to enteric dysfunction, one of the primary drivers of antimicrobial use in neonatal calves [ 41 ].
Despite having similar core functional structures, significant differences in the proportion of metabolic pathways were found between groups, particularly in groups C and D, which showed an increasing trend in pathways related to metabolic processes and biosynthetic functions, suggesting higher functional complexity compared to groups A and B.