Barely Significant

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The only marginally significant finding was a lower stranger-directed fear (SDF) score for Group 1A (singleton dogs with FDA score, had lived with other dogs previously) compared to Group 2 (singleton dogs with no FDA score; 0.364 ± 0.25 vs. 1.27 ± 0.23 respectively, W = 3.721, p = 0.042, Kruskal–Wallis with Dwass–Steel–Critchlow–Fligner pairwise comparisons).
highly significantqualifiedgold
The fluctated enzymatic activities revealed, the pathways including ferroptosis, pyrimidine metabolism, protein processing in endoplasmic reticulum have been significantly enriched, and the key metabolity, L-malic acid showed a highly significant positive correlation with the pathway of pyruvate cycle and citric acid cycle.