Barely Significant

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Concerning the variation in the solubility profile and the ζ of the native and modified proteins, the pI of the proteins presented an increasing trend for the modified proteins by N-cationization and N-acylation; however, they did not show statistically significant differences ( p- value > 0.05).
The concentration of demethyloleuropein, neohesperidin dihydrochalcone, and the chromen-4-one derivative showed an increasing trend throughout the developmental process of BYC, while the abundance of apigenin 7-sulfate declined from D150 to D300, and afterwards remained in a stable state from D300 to D450 ( Figure 5 ).