Barely Significant

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Independent measurements of the derivative reagent δ 2 H values are possible in some cases, but without accompanying correction for the amine-bound hydrogen in derivatized amino acids, errors in the reported δ 2 H values of amino acid carbon-bound hydrogen may be significant (on the order of 10 to 100‰; Supplementary Figure S7 ; Supplementary Section 4 ).
For isobutanol, only the EC1118a -SER33 knockout strain showed a decreasing trend and the effect was significant with a reduction of 71.346%; whereas for isoamyl alcohol, the EC1118a -ADH4 and EC1118a -TIR1 knockout strains showed a significant reduction of 21.003 and 17.334%, respectively.
3.3 High-throughput sequencing of 16S rDNA 3.3.1 Venn and α-diversity analysis of gut microbiota A Venn analysis showed a total of 679 operational taxonomic units (OTUs) in the two groups ( Figure 4A ), of which each group had unique OTUs, and the OTUs in the OVX group showed a decreasing trend compared with the sham group.
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3.7 Correlation analysis of soil carbon, nitrogen, and phosphorus cycling functional genes with environmental factors and functional microorganisms Heat map analysis of soil carbon, nitrogen and phosphorus cycling genes correlated with soil physicochemical properties and enzyme activities ( Figure 9 ), korA/B , which is associated with carbon fixation, was positively correlated with S-β-GC, and TC and SOM had high concordance, and negatively correlated with most of the carbon cycling genes ( Figure 9A ); GDH2 , norB were highly significant negatively correlated with TC, SOM, AN and S-LAP, glnA, nirB and gltB were all significantly negatively correlated with SOM, where gltB and AN were highly significantly negatively correlated, and nirA was the opposite, but TN showed a weak correlation with the nitrogen cycle; for the soil phosphoru
In general, the CP content in silage shows a decreasing trend as the anaerobic fermentation process advances, and the biological activity of adherent microorganisms affects the degree of degradation of CP content after the addition of exogenous additives ( He et al., 2018 ).
3.3 Volatility analysis Table 5 displays the volatility analysis results, with the correlation coefficients ( r -value) and corresponding significance ( p -values) of the relationships between the performance, digestibility and slurry variables studied and bacterial genera found to be significant or near to significance.