Barely Significant

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Thus, regarding all above described MGEs, an overall trend of higher MGE carriage in clinical than in non-clinical strains was recorded, while no specific MGE was connected to clinical background. 3.6 Pairwise genome comparisons with implementation of preceding analyses The previous analyses of accessory genes, ARGs, and MGEs showed differences between clinical and non-clinical strains.
Throughout the fermentation process, apart from the glucose content of the AC treatment that appeared an increasing trend in the first 7 days of ensiling, other fermentable carbohydrates decreased gradually with the extension of ensiling time, which decreased sharply in the first 7 or 14 days of ensiling and then decreased slowly.
In contrast, the proportions of propionate ( p = 0.549) and butyrate ( p = 0.056) had no significant change ( Figure 3F ), and the proportion of butyrate ( p = 0.056) showed an increasing trend. 3.4 Effects of STE treatment on the rumen bacterial community richness and diversity in weaned calves To explore how the supplementation of STE altered ruminal fermentation, DNA was extracted from ruminal digesta, and 16S rRNA gene sequencing was performed to study the changes in ruminal microbiota.