Barely Significant

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A clear trend was established; PAC mutants with low levels of enzyme activity such as Y171F generated very few colonies at low (1 μg/mL) puromycin concentrations and none at higher concentrations (3–5 μg/mL; Fig. 3 b), while PAC mutants with higher levels of enzyme activity such as Y30F, retained their ability to promote efficient colony formation across all puromycin concentrations used, albeit with fewer colonies than the WT (Fig. 3 b and Supplementary Table S4 ).
Additional NAFLD-specific phenotypes, such as inflammation and hepatocellular ballooning 31 , did not differ significantly between treatment groups in the samples analyzed, although there was a slight trend toward improved inflammation scores in the WD/LD-T group relative to the WD/WD-V group (Supplementary Table S2 ).