Barely Significant

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For isobutanol, only the EC1118a -SER33 knockout strain showed a decreasing trend and the effect was significant with a reduction of 71.346%; whereas for isoamyl alcohol, the EC1118a -ADH4 and EC1118a -TIR1 knockout strains showed a significant reduction of 21.003 and 17.334%, respectively.
3.3 High-throughput sequencing of 16S rDNA 3.3.1 Venn and α-diversity analysis of gut microbiota A Venn analysis showed a total of 679 operational taxonomic units (OTUs) in the two groups ( Figure 4A ), of which each group had unique OTUs, and the OTUs in the OVX group showed a decreasing trend compared with the sham group.
We found a clear trend of segregation of significantly different genera between the two groups, with stronger aggregation of significantly higher genera (green nodes) in the rumen of adult buffaloes and stronger aggregation among significantly higher genera (yellow nodes) in the rumen of breastfed calves, suggesting that we have a community effect and symbiotic relationship between microorganisms in the rumen of buffaloes at different growth stages ( Figure 4 ).
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3.7 Correlation analysis of soil carbon, nitrogen, and phosphorus cycling functional genes with environmental factors and functional microorganisms Heat map analysis of soil carbon, nitrogen and phosphorus cycling genes correlated with soil physicochemical properties and enzyme activities ( Figure 9 ), korA/B , which is associated with carbon fixation, was positively correlated with S-β-GC, and TC and SOM had high concordance, and negatively correlated with most of the carbon cycling genes ( Figure 9A ); GDH2 , norB were highly significant negatively correlated with TC, SOM, AN and S-LAP, glnA, nirB and gltB were all significantly negatively correlated with SOM, where gltB and AN were highly significantly negatively correlated, and nirA was the opposite, but TN showed a weak correlation with the nitrogen cycle; for the soil phosphoru
The exceptions, which were barely statistically significant, were (i) a shorter recurrence interval for isoclonal group A isolates (10 pairs: median interval, 45 days) than for isoclonal group C isolates (four pairs: median interval, 90.5 days) ( p = 0.02), and for fluoroquinolone-resistant isoclonal isolates (59 pairs: median interval, 56 days) than for fluoroquinolone-susceptible isoclonal isolates (60 pairs: median interval, 65 days) ( p = 0.04).