Barely Significant

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Interestingly, eight JAZ subfamily genes ( PaJAZ1 , PaJAZ6 , PaJAZ7 , PaJAZ8 , PaJAZ9 , PaJAZ11 , PaJAZ12 , and PaJAZ14 ) all showed an increasing trend from the S1 to S2 stage (peaking at the S2 stage), followed by a decreasing tendency from the S2 to S4 stage, with relative expression levels reaching their lowest level, except for PaJAZ12 , which was equal to the S2 stage.
The expression levels of spermatogonia markers dnd1 , stmn1 and zbtb16 ; spermatocytes markers ccne2 , ccnb1 and e2f2 ; and spermatid/spermatozoa marker cfap69 were not significantly affected ( p > 0.05), even though there was an elevated trend ( Figure 5 ). 2.6. psmd9 Knockdown and Its Effect on Other Genes We further performed a knockdown of psmd9 by RNAi.