Barely Significant

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Other osteogenic marker genes (ALPL, alkaline phosphatase, Figure 5 B; SPP1, osteopontin, Figure 5 C; and BGLAP, osteocalcin, Figure 5 D) showed a trend towards induction after stimulation with Sema3A beyond the effect of the osteogenic medium (mineralization medium: min. med. = DMEM, supplemented with 50 mg/mL ascorbic acid, 10 mM ß-glycerophosphate, 10-7 M dexamethasone).
At the same time, the BSP gene level in rDPSC-CellSiac and rBMSC-CellSaic cultured at 7, 14, and 21 days after osteogenic differentiation showed an increasing trend with time, where the BSP gene level at each time point was significantly higher than the respective level in the undifferentiated group.