Barely Significant

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We observed a clear trend that birds sang sooner at higher rates after longer LO durations: first song latencies monotonically decreased as LO duration increased (Fig. 2 B, C; n = 10 birds; p = 0.010, F (3,36) = 4.29, one-way ANOVA; when birds did not produce any songs during the 2-h post-LO period, the data of first song latency was assigned a time of 2 h [120 min]; see Methods ); likewise, initial singing rates monotonically increased as LO durations increased (Fig. 2 B, D; p = 0.0001, F (3,36) = 9.1; when birds did not produce any songs, the data of the initial singing rate was assigned zero).