Barely Significant

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Characterization of gene expression revealed an expected trend for both encapsulated and control spheroids—expression of pluripotency genes ( OCT4, SOX2, NANOG ) was high with cells in pluripotency (mTeSR) media at days 1 and 3 but then decreased after spheroids were transferred into endodermal differentiation media during subsequent 3 days (Fig. 6 B,C).
The results show that: (1) the annual runoff indicates an increasing trend in the future in contrast with the base period, while the ascending rate of the basin under RCP 4.5 is 4.47%; (2) a nonlinear relationship has been identified between the optimal allocation of water resources and water availability, while a linear association exists between the former and water demand; (3) increased water supply are needed in the water donor area, the middle and lower reaches should be supplemented with 4.495 billion m 3 water in 2030.