Barely Significant
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“did not reach statistical significance”

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did not reach statistical significancep = 0.0691.4× alphaplainly factual
The post hoc analysis indicated that the LE group demonstrated significantly elevated Sirt1 expression compared to the HE group ( Figure 8 A, p < 0.001) and showed a trend toward elevated expression relative to the CE group, though this difference did not reach statistical significance ( Figure 8 A, p = 0.069).
Furthermore, although the difference between the groups did not reach statistical significance, the IL-6 plasma levels showed a trend reduction over time after the FS was administered (pre–post treatment 8.0 ± 1.73 pg/mL vs. 4.8 ± 1.20 pg/mL) but not after the placebo was administered (pre–post 5.6 ± 0.94 pg/mL vs. 5.01 ± 1.07 pg/mL).
did not reach statistical significancep = 0.132.6× alphaplainly factual
The intensity of GLuc luminescence in rat blood tends to be higher in the IPP (+) group [IPP (−) vs IPP (+) (AU); 2600 ± 224 vs 2824 ± 241, p = 0.13, Cohen’s d = 0.96]: Although the difference did not reach statistical significance ( p = 0.13), the large effect size in the animal experience (Cohen’s d = 0.96) suggests a notable trend (Fig. 7j ).
Furthermore, the age at treatment onset showed a trend toward earlier initiation in the pandemic group (8.04 years) compared to the pre-pandemic group (8.65 years), although this difference did not reach statistical significance (p > 0.05).