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“showed a trend”

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Nevertheless, consistent with the results observed in the dye uptake assay, treatments with Bio-AgNP, C1, and C2 showed a trend toward increased leakage for both biomolecules, with numerically higher values compared to their respective control groups.
RNA analysis in MLO‐Y4 osteocytic cells exposed to H 2 O 2 ‐induced oxidative stress revealed a significant increase in Sost (the gene encoding sclerostin) expression, while Wnt signalling–related genes ( Axin2 , Lef1 and Ctnnb ) showed a trend of compensatory upregulation, possibly reflecting a feedback response (Figure S4 ).