Barely Significant

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More specifically, a consistent pattern emerged across both primer sets (universal prokaryotic and archaeal ones) indicating a notable reduction in methanogenic archaea from day 15 to day 45 in both feeding groups (Group 2 and Group 3), while the control group (Group 1) showed an increasing trend over the same period.
Compared with the control group, the LD concentration in the liver showed an increasing trend with increasing nitrite concentration on days 1, 3, 7, 14, 21, and 28 of stress, and the LD concentration in the liver of the spotted seabass was significantly ( p < 0.05) higher on days 3, 7, 14, 21, and 28 of nitrite stress. 3.7.
The tendency of RE to reduce BST provides direct evidence of its ability to alleviate thermal discomfort in heat-stressed dairy buffaloes, though it should be noted that this reduction only exhibited a statistical trend rather than a significant difference, which may be attributed to the relatively small sample size in the present study.