Barely Significant

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1-Hexanol, a VOC with an odor of freshly mown grass, exhibited a decreasing trend from day 0 to 21, apart from the PA-treated samples, which demonstrated an increasing trend. 1-Nonadecene was abundant in the control samples but present in lower quantities under LAB-treated conditions due to higher acidity, as evidenced by the detection of acetic acid in these samples but not in the control.
Both kefir water treatments, however, showed a trend toward reduced abundance of Lachnospiraceae NK4A136 group (Kefir+D-gal: 0.059 ± 0.023; pre-Kefir+D-gal: 0.030 ± 0.006) and Prevotellaceae UCG-001 (Kefir+D-gal: 0.037 ± 0.006; pre-Kefir+D-gal: 0.063 ± 0.031) compared to the D-gal-treated group ( Lachnospiraceae NK4A136: 0.113 ± 0.014, p = 0.125; Prevotellaceae UCG-001: 0.089 ± 0.010, p = 0.271).
faecium WEFA23 significantly increases mRNA expression of ADCY2 ( p < 0.05), CCL5 ( p < 0.05), as well as CXCL9 ( p < 0.001), and a decreasing trend of ORAI3 , while deficiency of the OTC abolished the regulatory effect and achieved a result similar to L. monocytogenes exposure.