Excerpts

At n = 150, we’re kind of back where we started, with a very non-significant p value.

In the Asian region, SSPs 1, 2 and 4 show an increasing trend in forest cover between 2015 and 2050, and SSPs 3 and 5 show a decreasing trend.

To understand whether SIRT3 is also involved in the adaptive responses of neuronal network activity to IF, we performed immunoblot analysis of SIRT3 protein levels in hippocampal tissues and found that 24 h of FD in AL control mice had no effect on SIRT3 levels, and that mice maintained on IF for 1 month exhibited a highly significant elevation of SIRT3 levels (Fig. 2a ).

GSEA with these CIZ1-dependent transcription units also revealed highly significant overlap with genes regulated by PRC1 and PRC2 (Fig. 3a , Supplementary data 2 , tab 1 ), though unlike culture-adapted cells there was little change in PRC subunit transcript levels (Supplementary Fig. 2 ), and also no gross change in the overall immuno-staining pattern for SUZ12, RING1B or BMI1 in primary cells (Supplementary Fig. 2 ).

Egger regression and MR-mode yielded substantially wider confidence intervals thus leading to statistically non-significant or borderline significant results.

The difference in CDI between ORAI1 and ORAI1β, which was highly significant when CRAC currents were activated by including 10 mM of the slow buffer EGTA in the pipette (Fig. 1b–d ) disappeared when CRAC currents were activated by dialysis of 20 mM of the fast chelator BAPTA through the pipette (Fig. 1e–g ) or when extracellular Ca 2+ was replaced by Ba 2+ (Fig. 1h–j ), strongly arguing that this difference in CDI is mediated by cytosolic Ca 2+ at the vicinity of ORAI1 channels.

Similarly, although altitudinal range is associated with language diversity at high resolution with marginal significance, there is no evidence that this is caused by isolation, as altitudinal range does not result in reduction in speaker population size, even when controlling for population density (Table 3 ).

However, while multiple nominally significant cis -eQTLs were present, nearly half of all loci had no evidence of cis -eQTLs in the sample sets used.

Clearly, R Ω , the resistance of the solution and electrode, presented a slightly increasing trend from 10.8 to 17.5 Ω/cm 2 under the potential of 1.2 to 1.6 V; meanwhile, the R ct, the charge transfer resistance, gave a decreasing trend from 277.4 to 168.6 Ω/cm 2 .

Conversely, in BACE1-overexpressing cells, Aβ34 levels were significantly elevated approximately threefold compared with APP-overexpressing conditions, whereas Aβ38, Aβ40, and Aβ42 levels showed only a mild trend toward elevation compared with the control (Fig. 3e–h ).

6b, c, f ), but not IL-6 or GM-CSF secretion, though a clear trend in reduction was observed (Fig. 6d, e ).

There was a highly significant correlation between senescent (TAF + ) cells in the interstitial dermis and increasing donor age (Fig. 7c ).

A positive trend for accumulation of T and B cells in tumors of Camkk2 −/− mice were also observed.

While this was expected for G and G + E models based on results from previous studies 21 , 23 , 24 , 26 , it was surprising for GxE SNPs, as these often do not have highly significant main genetic effects.

One mTOR-dependent phosphorylation site of 4EBP1 (Thr 37/46) was increased, while a second one (Ser 65) showed a trend of increase.

The correlation of caste bias and sex bias was not restricted to the abdomen, as there were similar highly significant effects when comparing transcriptomic profiles in the head and thoracic tissues, albeit with weaker effect sizes (Supplementary Fig. 7 ).

Interestingly, while α-ketoglutarate (α-KG) was reduced in cells overexpressing hIAPP, its intermediate 2-hydroxyglutarate (2-HG), which may divert glucose from entry into the TCA cycle 33 , showed a trend to be increased (Supplementary Fig. 6a ).

We also found modestly significant high-frequency-biases in the inputs from L3 and L5, but not L2 and L4 (See Supplementary Fig. 2 ).

The different colloid sizes in the precursors with different x values are confirmed by the dynamic light scattering (DLS) results (Supplementary Figure 3 ), which show a decreasing trend from around 163.0 nm ( x = 1.0) to around 3.6 nm ( x = 3.0).

There was a weak trend suggesting that a few glycosylation sites in the V2 hypervariable region may also be associated with sensitivity ( p -values of 0.01).

Despite the variation in the response of fibroblasts isolated from different patients, the pattern of response was highly significant and clearly consistent among all samples (i.e., induction of lipogenic marker expression and suppression of COL1A1 expression).

The results showed a trend for mild downregulation of the lipogenic markers PLIN2 (Fig. 5b ) and PPARg (Fig. 5c ) in parallel to a robust, significant downregulation of COL1A1 (Fig. 5d ).

At a regional scale, the correlation is positive, very strong and highly significant for the European region and moderately strong for China.

For edgeR (FDR adjusted, Fisher’s exact test in the edgeR R package), highly significant (top 100, top 200, top 300, top 400, and top 500) differential genes were selected.

The average score for each predicted function was calculated by normalising the scores from the individual methods (e.g., e -value or probability) to the range of 0–100, with 100 indicating a highly confident score (e.g., a highly significant e -value from Pfam or Gene3D; see methods).