Barely Significant

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Although demented individuals showed a trend toward lower microbial diversity, our results suggest that dementia-related alterations are characterized less by a global loss of diversity and more by specific compositional shifts, with depletion of beneficial taxa and enrichment of potentially harmful ones, resulting in a distinct microbial signature.
Western blot analysis showed that although the phosphorylation of AMPK (p-AMPK) exhibited an increasing trend in the MG5012- and MG741-treated groups compared to the HFD group, this difference did not reach statistical significance ( Figure 4 F,I).
Following the combined intervention, gut microbial diversity and community structure displayed a clear trend toward recovery to a normal state, suggesting that B. lactis Ca360 helps restore the disrupted intestinal ecological balance under iron-deficient conditions [ 59 ].