Barely Significant

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Searching for the conserved HeLo domains using the HMM also yielded a significant match to a HET-s/LopB domain from Metarhizium anisopliae ARSEF 23 [GI: 322703231, E-value 1.6e-10], as well as marginally significant matches [GI: 310797955, GI: 317157340, GI: 317033349] in several proteins from Glomerella graminicola , Aspergillus oryzae RIB40, and Aspergillus niger CBS 513.88, respectively [corresponding E-values 0.0042, 0.00082, 0.00083].
qRT-PCR data for GTPase RhoA ( Figure 3J ) and phosphatidylinositol 4-kinase ( Figure 3K ) showed a trend similar to that of the array data ( Figure 2A ), where GTPase RhoA showed fewer transcripts at the 4 or 12 h time points, and phosphatidylinositol 4-kinase showed lower mRNA expression at the 12 or 24 h time points in E 2 -treated samples compared to controls ( Figure 3J and 3K ). qRT-PCR also showed a significant increase in phosphatidylinositol 4-kinase transcript numbers at 24 hours of transition in controls compared to E 2 -treated samples ( Figure 3K ).