Excerpts

1c ), which exhibited highly significant hypomethylation ( P < 2.2e-16; Fig. 1d ).

239.3 cm) compared to Sb, and in both cases differences resulted highly significant ( p = 2.2×10 −16 and p = 2.7×10 −15 for MAT and PH, respectively).

These differences, while modest in strength, were highly significant (p < 2.2e-16).

The Wilcoxon–Mann–Whitney test confirmed that these differences were highly significant ( p -value < 2.2 × 10 −16 ). 3.3.

We observed a very weak but highly significant correlation in synonymous heterozygosity between species (Pearson’s r = 0.14, p<2.2e-16), consistent with some degree of conservation for genic parameters of mutation rates and/or selection at Drosophila synonymous sites [ 58 , 70 – 74 ].

We found a highly significant enrichment (Fig. 5 ; P < 2.2e-16) of small GWA P values for LD blocks with a sQTL in the German DCM GWAS (909 cases versus 2120 controls), which was subsequently replicated ( P < 2.2e-16) in the international DCM GWAS (1179 cases versus 1108 controls).

To test this prediction, we performed DRIP-seq in ovaries dissected from adult females and found strong, highly significant correlations between the female whole fly samples and ovary samples for both nonDE and FE peaksets from the adult data (Spearman’s rho > = 0.72 and P < 2.2e-16 in all comparisons, S2D Fig ).

83.36%, respectively), although the difference was highly significant (two-sided Brunner–Munzel paired-rank test, P < 2.2 × 10 −16 ).

This overall deficiency of heterozygotes was statistically highly significant ( t = 43.322, df = 33,234, p < 2.2e-16), and likely due to population subdivision (see results below).

As expected, we observed a highly significant difference between RefSeq mRNAs (NM) and RefSeq non-coding RNAs (NR) for both the CPC and the RNA-code score (CPC t = 56.4326 [2.239631;2.400904], p-value<2.2×10 −16 , RNAcode t = 39.6171 [34.44711;38.03595], p-value<2.2×10 16 ).

Notably, the correlations between differential patterns of H3K4me1/2/3 and H3K27me3 in promoters of genes that were up- or down-regulated in each spermatogonial subtype, respectively, were highly significant (p < 2.2 −16 ) ( Figures S3 A and S3B).

Consistent with this, many of these genes (80/173) were also downregulated in the DIG1 residual profile, a highly significant enrichment (Fisher’s exact test, 1-tailed: P < 2.2e−16).

We observed a highly significant deviation from the expected distribution (Chi-squared test, p < 2.2 × 10 –16 ), indicating strong cell-type-specific expression patterns.

2d and e ) and the Pearson correlation of these DNAm changes is highly significant ( p < 2.2 × 10 −16 ).

2 A), which was still highly significant ( p = 2.2e-16) due to the number of data points.

We find a highly significant correlation (ρ = 0.15, P < 2.2 × 10 −16 ) ( Supplemental Fig.

Although circRNA expression shows a clear trend for both positive (β = 1.121, P < 2.2 × 10 −16 ) and negative (β = −1.015, P < 2.2 × 10 −16 ) β values (effect size), there is no such trend in the mRNA expression (β = 0.0294, P = 0.8654; and β = −0.0339, P = 0.8639 for positive and negative β values, respectively).

In this study, we found a highly significant correlation between the letter and pseudo-letter versions of the MEP task ( r = 0.73, p < 2.2 × 10 −16 ; CI = 0.710–0.758; dis-attenuated correlation r = 0.915, with task reliability of 0.80).

These tests were all found to be statistically highly significant, with p < 2.2 × 10 −16 .

For 9 out of the 16 motifs we examined, the susceptibility of the vulnerable genes was strongly lower than that of the resistant gene sets to a similar degree, a difference that was highly significant (t-test, P < 2.2 × 10 −16 for all 9 motifs, Fig 5A columns shown in orange box).

The polarity score analysis of all expressed ORFs revealed a small but highly significant shift of ribosomes toward the 5′ end of ORFs when RPLP1/2 were depleted (mean of the differences = 0.013, P = 2.2 × 10 −16 ) ( Supplementary Figure S12A ).

This revealed a highly significant agreement of the number of events between paired replicates (Supplementary Fig. 2b , Pearson's correlation 0.997, n = 30, two-sided, p value < 2.2e−16), indicating that uvCLAP events capture the amount of pulled down RNA exceptionally well.

We further validated the relationship between UMOD and NKCC2, by Taqman qRT-PCR, showing a highly significant correlation of expression (p = 2.2e−16, R = 0.844) (n = 86).

This overlap is highly significant ( P < 2.2e-16), indicating that H3K4me3 levels at a core set of genes provide a fingerprint of overall health in both mothers and children at 1 y of age.

We find a highly significant departure from the expectation that the mean blunting score is equal to zero (two-tailed one-sample t test P < 2.2 × 10 −16 ), demonstrating that deletion of ENH5 results in a transcriptome-wide dampening of the response to sustained hypoxia ( Fig. 3E ).