showed a trendP > 0.05
Adenine intake decreased the diversity and separated microbiota structure Chao1, Observed_species, and Shannon indexes were significantly lower in the NMM group compared with the NC group ( P < 0.05; P < 0.05; P < 0.05), and the above indexes showed a trend of reduction in the NML group ( P > 0.05; P > 0.05; P > 0.05) ( Fig. 4 A–C), suggesting that 50 mg/(kg·d) adenine intake markedly reduced diversity of gut content microbiota of mice.