Barely Significant

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Accordingly, the average oxidation state of Ru for N 0.1 M 0.1 RO is calculated to be +3.79 for N 0.1 M 0.1 RO, +3.32 for N 0.1 M 0.1 RO 50 h@10 mA cm −2 , and +3.26 for N 0.1 M 0.1 RO 150 h@200 mA cm −2 , confirming that the average oxidation state of Ru exhibits a decreasing trend after the long-term stability measurement.
Over time, the expression of TBR1 decreased (28% ± 10%), whereas BRN2 (41% ± 5%) and SATB2 (40% ± 8%) showed an increasing trend, ultimately resulting in relatively balanced distribution of deep and superficial layer neurons within this system (Fig. 2a and Supplementary Fig. 4b ), in contrast to many differentiation protocols that result in reduced production of upper-layer neurons.