We performed immunoprecipitation-mass spectrometry (IP-MS) identification of N-terminal-tagged CLK2-associated proteins (4 FLAG-tagged replicates, one untagged CLK2), using tandem mass tags (TMT) mass spectrometry and observed highly significant enrichment (Fig. 6a , Supplementary Data 32 ) of both known CLK-interacting proteins (28/97 known, B-H adjusted Wald p < 0.05) including CLK1 and additional splicing factors (98/270 total enriched, B-H adjusted Wald p < 0.05).
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Markers with nominally significant p-values in one or more cell populations ( P ≤ 0.05; e.g CD39, CD38, Ki-67, PD-1) were visualized in boxplots; statistical significance computed using the linear mixed model were further confirmed using non-parametric Wilcoxon rank sum test.
A linear mixed effects model fitted to stimulus value ratings (i.e., how much participants wanted to eat the food items) found a main effect of group ( ß = 0.10, SE = 0.03, p = 0.003, 95% CI [0.03–0.16]), controlled for BMI, which had a borderline significant effect on stimulus value ratings ( ß = 0.01, SE = 0.006 p = 0.05, 95% CI [−0.0002 to 0.02]).
All signals show at least nominally significant ( P <0.05) directionally concordant associations in the follow-up studies of clinically validated PCOS cases, with no significant heterogeneity by PCOS case definition ( Supplementary Table 2 ).
In particular, the proportions were significantly higher for HIF1A and EIF5B compared to other genes, although the difference was only marginally significant ( P = 0.05; Permutation test; Fig. 4b ).
Embryos treated with (5Z)-7-Oxozeaenol showed a slight, but not quite significant (p = 0.05, Student’s t-test) increase in the percentage of cells expressing cleaved Caspase 3, suggesting that in these embryos another mechanism may be responsible for the decrease in extra-embryonic cells.
A significant trend for reduction in neutrophil counts and increase in T-lymphocytes with each DOL was observed in both cohorts (Fig. 2C ) by repeated measures ANOVA ( P < 0.05), and confirmed by flow cytometry (Supplementary Fig. 3 ).
Clinical information of these patients was provided in Supplementary Data 1 , with no significant differences in HBsAg, antiviral treatment, liver cirrhosis status, tumor number; modestly significant bias in gender, age and tumor size ( p < 0.05); and significantly lower microvascular invasion (MVI), Barcelona Clinic Liver Cancer (BCLC) stages and Edmondson-Steiner (E-S) grades in AFP − HCC versus AFP + HCC ( p < 0.001).
In total, 63 loci have shown at least marginal significance ( P <0.05) with ER-negative breast cancer.
Univariate linkage disequilibrium score regression (LDSC) 24 analysis showed nominally significant heritability for all 37 phenotypes ( P < 0.05, Table 1 ).
Nominally significant ( P < 0.05 but not meeting Bonferroni threshold P < 0.00238) included negative correlations with educational attainment and autism spectrum disorder and positive correlations with migraine, insomnia, and multiple sclerosis (Fig. 2a , and Supplementary Data 1 ).
coli lysates expressing IsmA and SpiR revealed that SpiR generated a higher cholestenone signal, with a marginally significant difference ( U = 9.0, p = 0.05, Mann–Whitney U test).
In total, we identified 31,162 nominally significant ( P value < 0.05, |r | >0.05) correlations between open chromatin and gene expression.
Although increased, the PF4 protein levels in platelets of young exercising mice (for 4 days) did not reach statistical significance when compared to standard-housed animals (2786 mean A.U. in standard-housed mice vs. 3969 mean A.U. in running mice; fold change: 1.42, p = 0.05, n = 5 mice per group).
Strikingly, there was a highly significant bias in the cluster corresponding to primary conjugated biliary acids at T1 (Fig. 4B , p < 0.05 Mann–Whitney) that was confirmed at T2 (Fig. 4C , p = 0.002 and p < 0.001 Mann–Whitney) in the nDC arm.
Using these phenotypes, we computed the odds ratio of the proportion of nominally significant ( p < 0.05) hits in the EPAS1 gene region (chr2:46,150,000–46,450,000) encompassing our regulatory elements compared with variants in a randomly selected matched window.
On the other hand, among the 986 SNPs with estimated PPR >99%, 47% were nominally significant with p < 0.05 in the replication cohort 23andMe, and 79% with consistent direction of effects.
None of these variants were genome-wide significant in either the sporadic or multiple consecutive miscarriage analysis, and only 14 (4.2%) and 11 (3%) were nominally significant ( P < 0.05) in the respective analyses (Supplementary Data 4 ), which is less than expected by chance (5%).
Thirty additional associations (24 positive, six inverse) were nominally significant ( p < 0.05).
We defined a variant’s eGFRcrea association as validated by eGFRcys/BUN when we observed a directionally consistent, nominally significant association with eGFRcys and/or BUN ( P < 0.05, same effect direction for eGFRcys and/or opposite effect direction for BUN).
31 by including as IVs only the eGFR-associated genetic variants ( p < 5 × 10 −8 ) with additionally having directionally consistent and nominally significant p-value (p < 0.05) with i) eGFRcys or ii) BUN.
In a replication study of the 18 novel loci, findings from the HF GWAS in the GBMI multi-ancestry excluding UK Biobank indicate 33.3% (6 of 18) of variants are significant ( p value <0.05/18), 61.1% (11 of 18) are nominally significant ( p value <0.05), and 100% have a beta estimate that is directionally concordant with our meta-analysis (Supplementary Data 20 ).
In 23andMe research cohort, 45 out of 51 lead SNPs available (88.2%) replicated with a consistent direction of effect at a Bonferroni corrected significance threshold of 9.8 × 10 −4 ( P -value = 0.05/51) and additional 2 SNPs were nominally significant ( P < 0.05) (Table 1 and Supplementary Fig. 4 ).
2a ), and 53 showed nominally significant associations in the CKDGen cohorts alone ( p < 0.05; Supplementary Data 4 ).
Statistics In all plots, only significant or at least marginally significant P values (significant level determined at p < 0.05) are specified.